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Proceedings Paper

Antibody fragment recognition layers for surface plasmon resonance biosensing: a parametric study
Author(s): André Magalhães; Sandro Bordeira; Ana Cristina Almeida; Vanessa Fontes; Maria João Leite Costa; Luís P. Fonseca; João Garcia da Fonseca
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Paper Abstract

A comparative study is reported regarding the use of two different surface plasmon resonance (SPR) biosensors, a homemade SPR grating biosensor and a reference prism coupled biosensor, to perform quantification of C-reactive protein (CRP) in human blood serum. Surface functionalization was conducted using anti-CRP fragments immobilized directly on gold. Adsorption time optimization for the antibody fragments monolayer, non-specific binding (NSB) resistance evaluation and CRP detection were conducted, with better results achieved by the grating biosensor on all topics, namely less functionalization time, higher resistance to NSB and wider CRP dynamic concentration range. A study regarding comparison between continuous flow and surface coating immobilization is also reported in this work. We have shown that surface coating immobilization achieves similar NSB resistance and CRP detection results, allowing a 75% assay cost reduction by lower solution volume requirement. Results suggest that the coating immobilization technique is the best suited to be used in further studies in order to obtain a viable immunosensor for CRP and other biomarkers detection in complex biological fluids.

Paper Details

Date Published: 23 February 2009
PDF: 8 pages
Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716903 (23 February 2009); doi: 10.1117/12.808820
Show Author Affiliations
André Magalhães, Biosurfit SA (Portugal)
Instituto Superior Técnico (Portugal)
Sandro Bordeira, Biosurfit SA (Portugal)
Ana Cristina Almeida, Biosurfit SA (Portugal)
Vanessa Fontes, Biosurfit SA (Portugal)
Maria João Leite Costa, Biosurfit SA (Portugal)
Luís P. Fonseca, Instituto Superior Técnico (Portugal)
João Garcia da Fonseca, Biosurfit SA (Portugal)

Published in SPIE Proceedings Vol. 7169:
Advanced Biomedical and Clinical Diagnostic Systems VII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D., Editor(s)

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