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Proceedings Paper

Tissue imaging of myocardial infarct regions by a slit-scanning Raman microscope
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Paper Abstract

Estimating the distribution of myocardial fibrosis after myocardial infarct is important for appropriate therapeutic planning. Here, we applied a Raman confocal microscope equipped with slit scanner for molecular tissue imaging of rat infarcted hearts. Raman spectra of the cytoplasm of cardiomyocytes included the resonance Raman bands at 751, 1130 and 1582 cm-1 arising mainly from reduced b- and c- type cytochromes. Raman spectra of fibrotic tissues at the borderzone of old myocardial infarct were highly consistent with that of collagen type I. Based on these findings, we successfully obtained Raman tissue images of a cardiomyocyte and surrounding collagen at the cellular level.

Paper Details

Date Published: 23 February 2009
PDF: 6 pages
Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 71690H (23 February 2009); doi: 10.1117/12.808079
Show Author Affiliations
Mitsugu Ogawa, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshinori Harada, Kyoto Prefectural Univ. of Medicine (Japan)
Yoshihisa Yamaoka, Kyoto Prefectural Univ. of Medicine (Japan)
Katsumasa Fujita, Osaka University (Japan)
Tetsuro Takamatsu, Kyoto Prefectural Univ. of Medicine (Japan)

Published in SPIE Proceedings Vol. 7169:
Advanced Biomedical and Clinical Diagnostic Systems VII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D., Editor(s)

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