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Proceedings Paper

Hardware-based anti-Brownian electrokinetic trap (ABEL trap) for single molecules: control loop simulations and application to ATP binding stoichiometry in multi-subunit enzymes
Author(s): Yan Jiang; Quan Wang; Adam E. Cohen; Nick Douglas; Judith Frydman; W. E. Moerner
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Paper Abstract

The hardware-based Anti-Brownian ELectrokinetic trap (ABEL trap) features a feedback latency as short as 25 μs, suitable for trapping single protein molecules in aqueous solution. The performance of the feedback control loop is analyzed to extract estimates of the position variance for various controller designs. Preliminary data are presented in which the trap is applied to the problem of determining the distribution of numbers of ATP bound for single chaperonin multi-subunit enzymes.

Paper Details

Date Published: 29 August 2008
PDF: 12 pages
Proc. SPIE 7038, Optical Trapping and Optical Micromanipulation V, 703807 (29 August 2008); doi: 10.1117/12.798093
Show Author Affiliations
Yan Jiang, Stanford Univ. (United States)
Quan Wang, Stanford Univ. (United States)
Adam E. Cohen, Stanford Univ. (United States)
Nick Douglas, Stanford Univ. (United States)
Judith Frydman, Stanford Univ. (United States)
W. E. Moerner, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7038:
Optical Trapping and Optical Micromanipulation V
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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