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Proceedings Paper

Photodynamic therapy of otitis media in-vitro and in-vivo using gerbil
Author(s): Chung-Ku Rhee M.D.; Pil Seung Kwon; Jin Chul Ahn; Phil Sang Chung; Ruifeng Ge
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Paper Abstract

The aim of this study was to evaluate antibacterial effects of PDT on common bacteria causing otitis media with effusion (OME). In vitro study was carried out using a hematoporphyrin derivative sensitizer (photogem) and 632 nm diode laser on H. influenzae, M. catarrhalis, and S. pneumoniae. One ml of each bacterial suspension was incubated for 3 hours and various concentrations of photogem were administered into the suspension. The suspensions were irradiated with 632 diode laser (15 J/cm2). The presence of colony forming units of the bacteria was examined, microscopic structures of bacteria were examined by TEM, and cytometry of bacteria was performed. The PDT was effective in killing all 3 kinds of bacteria. TEM showed damaged bacterial cell membrane and cytoplasmic structures and the flow cytometry showed lower number of viable bacteria in PDT group comparing to the control group. In vivo PDT study was performed using gerbil. S. pneumoniae or H. influenzae was injected into bullae. Photogem was injected into bullae in 2 days by when OME was developed and transcanal irradiation of 632 nm diode laser (90 J) was performed with a fiber perforated through an ear drum into a middle ear cavity and bulla. Four days after PDT, middle ear and bulla were washed with DPBS and the washed DPBS was cultured. The presence of bacterial colonies was examined. PDT was effective in killing S. pneumoniae in 87 % of the infected bullae with OME while it was effective to eradicate H. influenzae in 50 % of the infected bullae with OME. The results of these studies demonstrated that PDT may be effective to treat otitis media. It may have clinical implication to treat otitis media that is resistant to antibiotic therapy.

Paper Details

Date Published: 8 February 2008
PDF: 12 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422Y (8 February 2008); doi: 10.1117/12.774983
Show Author Affiliations
Chung-Ku Rhee M.D., Dankook Univ. (South Korea)
Pil Seung Kwon, Dankook Univ. (South Korea)
Jin Chul Ahn, Dankook Univ. (South Korea)
Phil Sang Chung, Dankook Univ. (South Korea)
Ruifeng Ge, Qingdao Municipal Hospital (China)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory M.D.; Reza S. Malek M.D.; Nikiforos Kollias; Brian Jet-Fei Wong M.D.; Henry Hirschberg M.D.; Justus F. R. Ilgner M.D.; Steen J. Madsen; Guillermo J. Tearney M.D.; Bernard Choi; Haishan Zeng, Editor(s)

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