Share Email Print

Proceedings Paper

Simultaneous imaging of two initiator caspases during cisplatin-induced HeLa apoptosis
Author(s): Jun Chu; Liang Wang; Qingming Luo; Zhihong Zhang D.V.M.
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Caspase-2 is an initiating caspase required for stress-induced apoptosis in various human cancer cells. Activation of caspase-9, a key event in stress-mediated apoptosis, also has been shown to be an initiator caspase. However, the timing or activation sequence of these initiator caspases, which trigger apoptotic pathway, is unclear. Here we report caspase-2 and caspase-9 dynamics during cisplatin-induced HeLa apoptosis using Double Fluorescence Resonance Energy Transfer (FRET) technique. Two FRET probes were constructed that each encoded a CRS (caspase-2 or caspase-9 recognition Site) fused with a cyan/yellow fluorescent protein (CFP/YFP) and a red fluorescent protein (DsRed) (CFP/YFP-CRS-DsRed). By using two probes, CFP-C2-DsRed and YFP-C9-DsRed, we carried out simultaneous double-FRET analysis and revealed that activation of caspase-2 had the same time course with caspase-9. These data suggest parallel activation of initiator caspase-2 and caspase-9 in cisplatin-induced cell death.

Paper Details

Date Published: 18 February 2008
PDF: 7 pages
Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570R (18 February 2008); doi: 10.1117/12.767864
Show Author Affiliations
Jun Chu, Huazhong Univ. of Science and Technology (China)
Liang Wang, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang D.V.M., Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 6857:
Biophotonics and Immune Responses III
Wei R. Chen, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?