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Proceedings Paper

Disruption of the blood brain barrier following ALA mediated photodynamic therapy
Author(s): Henry Hirschberg M.D.; Qian Peng; Francisco A. Uzal; David Chighvinadze; Michelle J. Zhang; Steen J. Madsen
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Paper Abstract

Introduction: Failure of treatment for high grade gliomas is usually due to local recurrence at the site of surgical resection indicating that a more aggressive form of local therapy, such as PDT, could be of benefit. PDT causes damage to tumor cells as well as degradation of the blood brain barrier (BBB). We have evaluated the ability of ALA mediated PDT to open the BBB in rats. This will permit access of chemotherapeutic agents to brain tumor cells remaining in the resection cavity wall, but limit their penetration into normal brain remote from the site of illumination. Materials and Methods: ALA-PDT was performed on non tumor bearing inbred Fisher rats at increasing fluence levels. T2 weighted MRI scans were used to evaluate edema formation and post-contrast T1 MRI scans were used to monitor the degree BBB disruption which could be inferred from the intensity and volume of the contrast agent visualized. Results. PDT at increasing fluence levels between 9J and 26J demonstrated an increasing contrast flow rate. No effect on the BBB was observed if 26J of light were given in the absence of ALA. A similar increased contrast volume was observed with increasing fluence rates. The BBB was found to be disrupted 2hrs. following PDT and 80-100% restored 72hrs later. Conclusion: PDT was highly effective in opening the BBB in a limited region of the brain. The degradation of the BBB was temporary in nature, opening rapidly following treatment and significantly restored during the next 72 hrs.

Paper Details

Date Published: 8 February 2008
PDF: 11 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422O (8 February 2008); doi: 10.1117/12.767323
Show Author Affiliations
Henry Hirschberg M.D., Beckman Laser Institute (United States)
Univ. of Nevada/Las Vegas (United States)
Qian Peng, The Norwegian Radium Hospital (Norway)
Francisco A. Uzal, University of California/Davis (United States)
David Chighvinadze, Univ. of Nevada/Las Vegas (United States)
Michelle J. Zhang, Univ. of Nevada/Las Vegas (United States)
Steen J. Madsen, Univ. of Nevada/Las Vegas (United States)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory M.D.; Reza S. Malek M.D.; Nikiforos Kollias; Brian Jet-Fei Wong M.D.; Henry Hirschberg M.D.; Justus F. R. Ilgner M.D.; Steen J. Madsen; Guillermo J. Tearney M.D.; Bernard Choi; Haishan Zeng, Editor(s)

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