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Proceedings Paper

Turn-key calibration of counter-propagating multiple beam 3D trapping system
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Paper Abstract

Optical trapping by use of multiple counter-propagating beam traps has not been widely implemented outside optical engineering laboratories. One, if not the primary, reason for this is the relatively complex calibration procedures involved in connection with this optical geometry. In this talk, we present automated solutions to all the calibration issues, which in effect results in a turn-key counter-propagating multi-beam 3D trapping system. These results allow a wider audience to utilize counter-propagating beam trapping systems. The calibrated system can be used to independently manipulate a plurality of cells real-time in a large 3D working area. Optionally, the system can be extended to allow for use of various spectroscopic methods concurrently with optical manipulation/trapping.

Paper Details

Date Published: 5 March 2008
PDF: 8 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68590Z (5 March 2008); doi: 10.1117/12.763096
Show Author Affiliations
Jeppe Seidelin Dam, Technical Univ. of Denmark (Denmark)
Ivan R. Perch-Nielsen, Technical Univ. of Denmark (Denmark)
Darwin Palima, Technical Univ. of Denmark (Denmark)
Jesper Glückstad, Technical Univ. of Denmark (Denmark)

Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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