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Proceedings Paper

Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy
Author(s): Tsung-Jen Wang; Wen Lo; Chen-Yuan Dong; Fung-Rong Hu
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Paper Abstract

The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.0D) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

Paper Details

Date Published: 11 March 2008
PDF: 7 pages
Proc. SPIE 6844, Ophthalmic Technologies XVIII, 684414 (11 March 2008); doi: 10.1117/12.762212
Show Author Affiliations
Tsung-Jen Wang, Taipei Medical Univ. Hospital (Taiwan)
National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Fung-Rong Hu, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6844:
Ophthalmic Technologies XVIII
Bruce E. Stuck; Fabrice Manns; Per G. Söderberg; Michael Belkin M.D.; Arthur Ho, Editor(s)

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