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Proceedings Paper

Characterization of cells and bacteria by photophoretic velocimetry
Author(s): Clemens Helmbrecht; Reinhard Niessner; Christoph Haisch
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Paper Abstract

The migration induced by intensive light is termed photophoresis. We could show that the evaluation of light-induced velocities of microparticles, bacteria and cells suspended in water is valuable for the prediction of their intrinsic properties. Two different laser setups were evaluated for photophoretic migration, a He-Ne laser (P = 45 mW, λ = 633 nm) and a diode-pumped cw-Nd:YAG (P = 1.1 W, λ = 532 nm). When analyzing the migration behavior of particles, we find significant differences depending on both, geometrical size and refractive index. We describe migration of PS particles of different size as well as with different refractive index but same diameter, SiO2 and melamine resin. The potential for the separation of biological matter is shown as velocity distributions of heat killed bacteria of Escherichia coli, Salmonella enteritidis, and baker's yeast is reported.

Paper Details

Date Published: 29 February 2008
PDF: 7 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 685913 (29 February 2008); doi: 10.1117/12.762095
Show Author Affiliations
Clemens Helmbrecht, Technische Univ. München (Germany)
Reinhard Niessner, Technische Univ. München (Germany)
Christoph Haisch, Technische Univ. München (Germany)

Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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