Share Email Print

Proceedings Paper

The influence of photodynamic therapy (PDT) with δ-aminolevulinic acid (ALA) on J-774A.1 macrophage cell line
Author(s): Aleksandra Kawczyk-Krupka M.D.; Zenon Czuba; Aleksandra Ledwon M.D.; Wojciech Latos; Ewelina Sliszka M.D.; Marta Mianowska; Wojciech Krol; Aleksander Sieron M.D.
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Introduction. The whole mechanism of the cellular level of tumor destruction by photodynamic therapy (PDT) is still unknown. Despite necrotic and apoptotic ways of cell death, there is a variety of events leading to and magnifying the inactivation of tumor cells. Material and methods. J-774A.1 were incubated with δ-aminolevulinic acid (ALA) at different concentrations (125, 250, 500, 1000 μM) and then irradiated with VIS (400 - 750 nm) at the dose of 5,10 and 30 J/cm2 delivered from the incoherent light source. The effects of the application of ALA-PDT were evaluated on the basis of cell viability, nitric oxide (NO), tumor necrosis factor α- (TNF-α) and interleukin-1β (IL-1β) produced by the J-774A.1 cells. Results. The cell viability (assessed using MTT test) was comparable with control group at 5,10 and 30 J/cm2. At these doses of energy using different concentrations of ALA we have observed that at the higher energy doses, the greater increase of TNF-α release, lowering of the level of IL-1β production and decrease of NO release were observed. There was also observed the dependence of the secretional activity of the cells on the ALA concentrations. Conclusion. The cell viability and production of cytokines depended on ALA concentrations and energy doses of the light. The higher some cytokines' release after PDT could be an additional factor for the complete eradication of tumor.

Paper Details

Date Published: 6 February 2008
PDF: 8 pages
Proc. SPIE 6857, Biophotonics and Immune Responses III, 685708 (6 February 2008); doi: 10.1117/12.762062
Show Author Affiliations
Aleksandra Kawczyk-Krupka M.D., Medical Univ. of Silesia (Poland)
Zenon Czuba, Medical Univ. of Silesia (Poland)
Aleksandra Ledwon M.D., Medical Univ. of Silesia (Poland)
Wojciech Latos, Medical Univ. of Silesia (Poland)
Ewelina Sliszka M.D., Medical Univ. of Silesia (Poland)
Marta Mianowska, Medical Univ. of Silesia (Poland)
Wojciech Krol, Medical Univ. of Silesia (Poland)
Aleksander Sieron M.D., Medical Univ. of Silesia (Poland)

Published in SPIE Proceedings Vol. 6857:
Biophotonics and Immune Responses III
Wei R. Chen, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?