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Proceedings Paper

Sensitivity and robustness of methods for analyzing time-resolved fluorescence measurements of layered biological tissue
Author(s): Anant Agrawal; Camisha Parker; Tariq Qazi; Krishan Agrawal; Joshua Pfefer
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Paper Abstract

Time-resolved fluorescence (TRF) measurements from layered biological tissue provide chemical and structural information which may be useful for imaging or single-point tissue diagnostics. While several techniques for analyzing TRF data have been proposed in the literature, a rigorous theoretical evaluation of these approaches has not been performed. In this study we have evaluated the sensitivity and robustness of four methods for analyzing TRF signals: biexponential deconvolution, single exponential deconvolution, Laguerre deconvolution, and direct peak width computation. Each of these analyses was performed on a large dataset of synthetic fluorescence decay curves. Each decay curve was generated by numerically convolving a pre-recorded nitrogen laser pulse with a biexponential decay based on fluorescence lifetimes of colonic mucosa. The relative contribution of each mucosal layer to the total TRF signal as well as the superficial layer's inherent lifetime were varied so as to investigate sensitivity to morphological and biochemical changes representative of a neoplastic disease process. To evaluate robustness, pre-set levels of Gaussian-distributed noise were added to the convolved curves to achieve variations in the signal-to-noise ratio. The relative merits and pitfalls of each analytical method are discussed.

Paper Details

Date Published: 6 February 2007
PDF: 8 pages
Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643022 (6 February 2007); doi: 10.1117/12.717654
Show Author Affiliations
Anant Agrawal, U.S. Food and Drug Administration (United States)
Camisha Parker, U.S. Food and Drug Administration (United States)
Virginia State Univ. (United States)
Tariq Qazi, Virginia State Univ. (United States)
Krishan Agrawal, Virginia State Univ. (United States)
Joshua Pfefer, U.S. Food and Drug Administration (United States)

Published in SPIE Proceedings Vol. 6430:
Advanced Biomedical and Clinical Diagnostic Systems V
Ramesh Raghavachari; Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)

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