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Proceedings Paper

Detection of cell metabolism via wavelength- and time-resolved intracellular autofluorescence
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Paper Abstract

A time-resolved confocal fluorescence spectroscopy system was instrumented utilizing the multi-channel time-correlated single photon counting (TCSPC) technique. The system provided a unique approach to investigate the relationship between wavelength- and time-resolved cell autofluorescence and cellular metabolic status. The experiments were carried out on monolayered cell cultures including normal and cancer ectocervical cells. With UV excitation at 365 nm, the decay of cellular fluorescence can be well described by a dual-exponential function, consisting of a short lifetime component ((tau)1~ 0.40 - 0.47 ns) and a long lifetime component (((tau)2 ~ 3.3 - 4.0 ns). By analyzing the decay-associated spectra of the short and long lifetime components, we found that the long lifetime component carried the information of protein-bound NADH and short lifetime component was mainly determined by free NADH with certain interference from bound NADH. Moreover, it was found that the ratio of the amplitudes of two lifetime components, dominated by free/bound NADH, was sensitive to cell metabolism. Overall, this study demonstrated that wavelength- and timeresolved autofluorescence can be potentially used as an important contrast mechanism to detect epithelial pre-cancer.

Paper Details

Date Published: 6 February 2007
PDF: 6 pages
Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 64300A (6 February 2007); doi: 10.1117/12.702459
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Wei Zheng, Hong Kong Univ. of Science and Technology (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)

Published in SPIE Proceedings Vol. 6430:
Advanced Biomedical and Clinical Diagnostic Systems V
Ramesh Raghavachari; Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)

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