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Proceedings Paper

In vivo imaging flow cytometer
Author(s): Clemens Alt; Ho Lee; Costas Pitsillides; Mehron Puoris'haag M.D.; Charles P. Lin
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Paper Abstract

Purpose: An in vivo flow cytometer was developed recently, providing quantification of fluorescently labeled cells in live animals without extracting blood samples. This non-invasive procedure allows continuously tracking a cell population of interest over long periods of time to examine its dynamic changes in the circulation. However, it has not been shown that counting signals arise from individual cells. Furthermore, cell morphology and cell-cell interaction in the blood stream (e.g. aggregation) are not visualized. Here we describe an imaging in vivo flow cytometer. Material and Methods: Fluorescence images are obtained simultaneously with quantitative information on a DiD-labeled cell population. As fluorescent cells pass through the slit of light focused across a blood vessel, the excited fluorescence is detected confocally. This cell counting signal triggers a strobe beam and an intensified CCD camera to capture a snapshot image of the cell as it moves down-stream from the slit. Results: Nearly all peaks counted as circulating T-cells originate from individual cells, while cell aggregates were rarely observed (<2%). Counting signal amplitude variation is attributed to uneven dye-loading among cells. We identify non-T-cells by their abnormal shape and size. Cell velocity was measured by determining the traveled distance from the slit within the delay of the strobe pulse or by applying multiple strobe pulses during the integration time of the CCD camera. Conclusions: An improved in vivo imaging flow cytometer can be a useful tool for studying cell populations in circulation.

Paper Details

Date Published: 19 February 2007
PDF: 7 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410I (19 February 2007); doi: 10.1117/12.702180
Show Author Affiliations
Clemens Alt, Wellman Ctr. for Photomedicine (United States)
Tufts Univ. (United States)
Ho Lee, Wellman Ctr. for Photomedicine (United States)
Kyongpook National Univ. (South Korea)
Costas Pitsillides, Wellman Ctr. for Photomedicine (United States)
Boston Univ. (United States)
Mehron Puoris'haag M.D., Wellman Ctr. for Photomedicine (United States)
Charles P. Lin, Wellman Ctr. for Photomedicine (United States)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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