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Proceedings Paper

Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy
Author(s): Stephen E. D. Webb; Selene K. Roberts; Sarah R. Needham; Daniel J. Rolfe; Chris J. Tynan; Marisa Martin-Fernandez
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Paper Abstract

Quantification of the oligomerisation state and conformational changes of the epidermal growth factor receptor in its constitutive and active states are critical to fully understand their signal transduction pathway. We show the ability of single-molecule fluorescence microscopy to determine this information and its implementation by combining multidimensional emission optics with total internal reflection fluorescence microscopy. Importantly, the signalling process is analysed in live cells under physiological conditions.

Paper Details

Date Published: 19 February 2007
PDF: 7 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410E (19 February 2007); doi: 10.1117/12.701417
Show Author Affiliations
Stephen E. D. Webb, CCLRC Daresbury Lab. (United Kingdom)
Selene K. Roberts, CCLRC Daresbury Lab. (United Kingdom)
Sarah R. Needham, CCLRC Daresbury Lab. (United Kingdom)
Daniel J. Rolfe, CCLRC Daresbury Lab. (United Kingdom)
Chris J. Tynan, CCLRC Daresbury Lab. (United Kingdom)
Marisa Martin-Fernandez, CCLRC Daresbury Lab. (United Kingdom)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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