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Proceedings Paper

Broadband coherent anti-Stokes Raman scattering (CARS): a potential tool for atherosclerosis diagnostic imaging
Author(s): J.-F. Cormier; A. Ko; L.-P. Choo-Smith; J. Werner; E. Kohlenberg; M. Hewko; J. Fréchette; M. Fortin; I. Noiseux; J. P. Bouchard; David Gay; P. Desroches; Francois Roy-Moisan; Marie-Eve Bourgault; M. G. Sowa; M. L. Vernon
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Paper Abstract

Nonlinear optical imaging technologies offer some intriguing medical diagnostic applications. Examples include fast imaging of elastin and collagen distributions in diseased tissues using two-photon fluorescence (TPF) and second harmonic generation (SHG), respectively. The 3D sectioning capabilities and biochemical specificity that enable fast imaging in highly scattering biological media lie at the heart of the appeal of these nonlinear approaches for medical applications. One of these promising nonlinear techniques relies on the resonance enhancement of the third order nonlinear susceptibility by a vibrational mode of a molecule. Coherent Anti-Stokes Raman Scattering (CARS) can provide similar vibrational information as a spontaneous Raman spectrum. The technique has been shown to be orders of magnitude more sensitive than spontaneous Raman, with video rate imaging demonstrated recently. In this work, we investigate the potential use of broadband CARS spectroscopy and CARS imaging for biochemical analysis of arterial tissue. Biochemical imaging data from broadband CARS is compared with spontaneous Raman microspectroscopy. The broadband CARS system comprised of a single femtosecond-laser is presented in detail. Issues related to data analysis, the advantages and current limitations of the CARS technique in biodiagnostics are discussed.

Paper Details

Date Published: 23 March 2007
PDF: 12 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64241X (23 March 2007); doi: 10.1117/12.701243
Show Author Affiliations
J.-F. Cormier, National Optics Institute (Canada)
A. Ko, National Research Council Canada (Canada)
L.-P. Choo-Smith, National Research Council Canada (Canada)
J. Werner, National Research Council Canada (Canada)
E. Kohlenberg, National Research Council Canada (Canada)
M. Hewko, National Research Council Canada (Canada)
J. Fréchette, National Optics Institute (Canada)
M. Fortin, National Optics Institute (Canada)
I. Noiseux, National Optics Institute (Canada)
J. P. Bouchard, National Optics Institute (Canada)
David Gay, National Optics Institute (Canada)
P. Desroches, National Optics Institute (Canada)
Francois Roy-Moisan, National Optics Institute (Canada)
Marie-Eve Bourgault, National Optics Institute (Canada)
M. G. Sowa, National Research Council Canada (Canada)
M. L. Vernon, National Optics Institute (Canada)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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