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Proceedings Paper

An in-vitro model for retinal laser damage
Author(s): Michael L. Denton; Michael S. Foltz; Kurt J. Schuster; Larry E. Estlack; Harvey M. Hodnett; Gary D. Noojin; Robert J. Thomas
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Paper Abstract

Ocular laser exposures resulting in damage at the retina typically involve cellular alterations in the retinal pigment epithelial (RPE) layer. To provide guidelines for eye-safe exposure to lasers, the laser safety community has relied on damage assessment in nonhuman primate studies. Simple and reliable model systems for laser bioeffects that use cultured RPE cells, rather than animals, are thus desirable. We have characterized our artificially pigmented hTERT-RPE1 model by identifying ED50 thresholds over a wide range of laser parameters and cell culture conditions. When summarized as action spectra and temporal action profiles (log threshold fluence versus log exposure duration), trends (pigment-dependent) in our cell model data are strikingly similar to the threshold trends reported for animal models (literature). In addition, the rapidity and flexibility (laser delivery) with which studies are performed in our culture model has benefited computational modeling efforts.

Paper Details

Date Published: 21 February 2007
PDF: 9 pages
Proc. SPIE 6435, Optical Interactions with Tissue and Cells XVIII, 643515 (21 February 2007); doi: 10.1117/12.701067
Show Author Affiliations
Michael L. Denton, Northrop Grumman (United States)
Michael S. Foltz, Northrop Grumman (United States)
Kurt J. Schuster, Northrop Grumman (United States)
Larry E. Estlack, Conceptual MindWorks, Inc. (United States)
Harvey M. Hodnett, Northrop Grumman (United States)
Gary D. Noojin, Northrop Grumman (United States)
Robert J. Thomas, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 6435:
Optical Interactions with Tissue and Cells XVIII
Steven L. Jacques; William P. Roach, Editor(s)

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