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Proceedings Paper

Semiconductor light-emitting devices with in-built bioreaction chambers
Author(s): H. D. Summers; D. R. Matthews; R. J. Errington; K. L. Njoh; P. J. Smith; A. D. Goater; J. P. H. Burt; N. H. Rizvi; A. Menachery; D. J. Morris
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Paper Abstract

We demonstrate complete integration of a fluorescence-based assay in that the analyte well is also an optical emitter. Laser machining is used to create 'active micro-wells' within semiconductor light emitting diode and laser structures. These are then used to optically excite fluorescently-labelled beads in solution within the well. The results show efficient illumination on a par with traditional lamp-based excitation. This technology therefore provides active microwell plates with completely localized excitation, confined to the analysis well, that can be engineered via the micro-well geometry. The micro-wells have also been machined within the cavity of lasing semiconductor structures and coherent emission maintained. Thus lasing multi-well plates are also realizable.

Paper Details

Date Published: 19 February 2007
PDF: 9 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410Y (19 February 2007); doi: 10.1117/12.700411
Show Author Affiliations
H. D. Summers, Cardiff Univ. (United Kingdom)
D. R. Matthews, Cardiff Univ. (United Kingdom)
R. J. Errington, Cardiff Univ. (United Kingdom)
K. L. Njoh, Cardiff Univ. (United Kingdom)
P. J. Smith, Cardiff Univ. (United Kingdom)
A. D. Goater, Univ. of Wales, Bangor (United Kingdom)
J. P. H. Burt, Univ. of Wales, Bangor (United Kingdom)
N. H. Rizvi, Univ. of Wales, Bangor (United Kingdom)
A. Menachery, Univ. of Wales, Bangor (United Kingdom)
D. J. Morris, Univ. of Wales, Bangor (United Kingdom)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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