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Proceedings Paper

Fluorescence tomography in a murine model of Alzheimer’s disease
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Paper Abstract

Noninvasive molecular imaging of amyloid plaques in murine Alzheimer's disease models would accelerate drug development and basic Alzheimer's research. Amyloid plaques differ from traditional fluorescent targets in size and spatial distribution and therefore present a unique challenge for biomarker development and tomography. To study imaging feasibility and establish biomarker criteria, we developed a digital mouse head model from a 100 &mgr;m-resolution, digital, segmented mouse atlas1. The cortical region of the brain was filled with a spatially uniform distribution of plaques that had different fluorescent properties from the surrounding brain tissue, similar to current transgenic mouse models of Alzheimer's disease. Fluorescence was simulated with a Monte Carlo algorithm using different plaque densities, detection geometries, and background fluorescence. Our preliminary results demonstrated that shielding effects might require nonlinear reconstruction algorithms and that background fluorescence would seriously hinder quantitative burden estimation. The Monte Carlo based approach presented here offers a powerful way to study the feasibility of non-invasive imaging in murine Alzheimer's models and to optimize experimental conditions.

Paper Details

Date Published: 13 February 2007
PDF: 8 pages
Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64342H (13 February 2007); doi: 10.1117/12.700199
Show Author Affiliations
Scott B. Raymond, Harvard-MIT Division of Health Sciences and Technology (United States)
Anand T. N. Kumar, Massachusetts General Hospital (United States)
Andrew K. Dunn, Univ. of Texas at Austin (United States)
David A. Boas, Massachusetts General Hospital (United States)
Brian J. Bacskai, Massachusetts General Hospital (United States)

Published in SPIE Proceedings Vol. 6434:
Optical Tomography and Spectroscopy of Tissue VII
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva Marie Sevick-Muraca, Editor(s)

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