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Proceedings Paper

Noninvasive imaging of transgenic GFP expression in neonatal mouse brain
Author(s): Gideon Ho; Chunyan Zhang; Lang Zhuo
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Paper Abstract

Glial fibrillary acidic protein (GFAP) is a traditional biomarker for astrocytes of the central nervous system. In this study, non-invasive in vivo imaging of GFAP-GFP (green fluorescent protein) expression in the brain of neonatal transgenic mice is used as a novel method to investigate the relationship between the expression of the transgene at 0, 2, 4, 6 and 8 hr post-treatment in mice subjected to a single administration of 12 mg/kg of neurotoxin 1-methyl-4(2'-methylphenyl)-1,2,3,6-tetrahydropyridine (2'-CH3-MPTP). The GFP elevation was found to peak at 6 hr and lasted to at least 8 hr after the toxin treatment. Histological examination of fixed brain sections using immunohistochemistry (IHC) shows an increase in GFP and GFAP signal from the substantia nigra pars compacta (SNpc) and the hippocampus. The results have provided quantitative fluorescence and qualitative histological evidence for the activation of the GFAP-GFP transgene in astrocytes following neurotoxin 2'-CH3-MPTP administration, suggesting that the model described here could be used to study neuronal degeneration such as Parkinson's disease and in general, developmental neurotoxicity in live animals.

Paper Details

Date Published: 23 March 2007
PDF: 12 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 642425 (23 March 2007); doi: 10.1117/12.700066
Show Author Affiliations
Gideon Ho, Institute of Bioengineering and Nanotechnology (Singapore)
Chunyan Zhang, Institute of Bioengineering and Nanotechnology (Singapore)
Lang Zhuo, Institute of Bioengineering and Nanotechnology (Singapore)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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