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Proceedings Paper

Ultra-short pulses to signal neuronal growth cone machinery
Author(s): Manoj Mathew; Ivan Amat-Roldan; Rosa Andres; Iain G. Cormack; David Artigas; Eduardo Soriano; Pablo Loza-Alvarez
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Paper Abstract

Measurable change in the sensory motor machinery of growth cones are induced by non contact femtosecond laser. The focused laser beam with an average power of 3 mW was positioned at some distance away from the closest fillopodia of cortical neurons from primary cell cultures (mice E15). By identifying a set of preliminary parameters we were able to statistically analyze the phenomenological behavior of the fillopodia and classify the effects different conditions of laser light has on the growth cone. Results show that fillopodia become significantly biased towards the focused femtosecond laser light. The same experiment performed with continuous wave (CW) produced results which were indistinguishable from the case where there is no laser light present (placebo condition) indicating no clear effects of the CW laser light on the fillopodia at a distance. These findings show the potential for ultrashort pulsed light to become a new type of pathfinding cue for neuronal growth cones.

Paper Details

Date Published: 19 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410G (19 February 2007); doi: 10.1117/12.699988
Show Author Affiliations
Manoj Mathew, Institut de Ciències Fotòniques (Spain)
Ivan Amat-Roldan, Institut de Ciències Fotòniques (Spain)
Rosa Andres, Parc Cientific de Barcelona (Spain)
Iain G. Cormack, Institut de Ciències Fotòniques (Spain)
David Artigas, Univ. Politècnica de Catalunya (Spain)
Eduardo Soriano, Parc Cientific de Barcelona (Spain)
Pablo Loza-Alvarez, Institut de Ciències Fotòniques (Spain)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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