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Proceedings Paper

X-ray and optical multimodality tomographer for small animal examination
Author(s): A. Da Silva; M. Leabad; T. Bordy; J.-M. Dinten; P. Peltié; P. Rizo
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Paper Abstract

A small animal multimodality tomographer dedicated to the co-registration of fluorescence optical signal and X-rays measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility to get in vivo anatomical and functional information at once. Moreover, anatomical measurements can be used as a regularization factor in order to get the reconstructions of the biodistribution of fluorochromes more accurate and to speed up the treatment. The optical system is basically composed with a CW laser (Krypton, 752 nm) for an optimal excitation of Alexa-Fluor 750 fluorochromes, and a CCD camera coupled with a combination of filters for the fluorescence detection. The animal is placed inside a transparent tube filled with an index matching fluid. In order to perform multiple views of fluorescence data acquisitions, the cylinder is fixed to a rotating stage. The excitation beam is brought to the cylinder via two mirrors mounted on translation plates allowing a vertical scan. The optical data acquisitions are performed with a high sensitivity CCD camera. The X-ray generator and the X-ray detector have been placed perpendicularly to the optical chain. A first study on phantoms was conducted to evaluate the feasibility, to test the linearity and the reproducibility, and to fix the parameters for the co-registration. These test experiments were reproduced by considering mice in the oesophagus of which the previous tubes were inserted. Finally, the performance of the system was evaluated in vivo on mice bearing tumours in the lungs, tagged with Transferrin-AlexaFluor 750.

Paper Details

Date Published: 8 February 2007
PDF: 8 pages
Proc. SPIE 6431, Multimodal Biomedical Imaging II, 64310R (8 February 2007); doi: 10.1117/12.699863
Show Author Affiliations
A. Da Silva, LETI-CEA MINATEC (France)
M. Leabad, LETI-CEA MINATEC (France)
T. Bordy, LETI-CEA MINATEC (France)
J.-M. Dinten, LETI-CEA MINATEC (France)
P. Peltié, LETI-CEA MINATEC (France)
P. Rizo, LETI-CEA MINATEC (France)

Published in SPIE Proceedings Vol. 6431:
Multimodal Biomedical Imaging II
Fred S. Azar, Editor(s)

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