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Proceedings Paper

Spatial difference in pH in Lucilia cuprina (Diptera: Calliphoridae) pericardial cells
Author(s): Osu Lilje; Erna S. Lilje
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Paper Abstract

Lucilia cuprina pericardial cells are primarily involved in the filtration of hemolymph. Ratio images using fluorescent pH indicator, DM-Nerf, were collected using a confocal microscope. The results support suggestions that there is zonation of cellular activity that reflect organelle distribution. Statistical analysis of the excitation ratios indicate significant spatial differences in pH of the three major zones- cortex, vacuole zone and endoplasm in pericardial cells. The outer cortex was estimated to have a pH between 5.5 and 6.8, the vacuole zone between 4.5 and 5.5 and the endoplasm between 4.0 and 5.0.

Paper Details

Date Published: 19 February 2007
PDF: 9 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411Q (19 February 2007); doi: 10.1117/12.699793
Show Author Affiliations
Osu Lilje, Univ. of Sydney (Australia)
Erna S. Lilje, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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