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Proceedings Paper

Optical tweezers force calibration using a fast shuttering camera
Author(s): J. P. Sharpe; Claudia Iniguez-Palomares; Rafael Jimenez-Flores
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Paper Abstract

We report using a fast-shuttering CCD camera to determine the transverse stiffness of an optical tweezers trap. By utilizing the relation between the particle position variance and the trap stiffness we are able to circumvent the sampling requirements of the position power spectrum approach. We find that for trap stiffness of order 10-5 N/m exposures shorter than 0.001 sec seem to be required in order to give valid measurements. For longer exposures (~0.01 sec) the trap stiffness can be overestimated by a factor of three or more.

Paper Details

Date Published: 13 February 2007
PDF: 8 pages
Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644114 (13 February 2007); doi: 10.1117/12.696272
Show Author Affiliations
J. P. Sharpe, Cal Poly State Univ. (United States)
Claudia Iniguez-Palomares, Ctr. de Investigación en Alimentación y Desarrollo (Mexico)
Rafael Jimenez-Flores, Cal Poly State Univ. (United States)

Published in SPIE Proceedings Vol. 6441:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V
Daniel L. Farkas; Robert C. Leif; Dan V. Nicolau, Editor(s)

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