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Proceedings Paper

Detection of colorectal cancer using time-resolved autofluorescence spectrometer
Author(s): Sheng Fu M.D.; Leong-Chuan Kwek; Teck-Chee Chia; Chu-Sing Lim; Choong-Leong Tang; Wuan-Suan Ang; Miao-Chang Zhou; Po-Ling Loke
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Paper Abstract

As we know Quantum mechanics is a mathematical theory that can describe the behavior of objects that are at microscopic level. Time-resolved autofluorescence spectrometer monitors events that occur during the lifetime of the excited state. This time ranges from a few picoseconds to hundreds of nanoseconds. That is an extremely important advance as it allows environmental parameters to be monitored in a spatially defined manner in the specimen under study. This technique is based on the application of Quantum Mechanics. This principle is applied in our project as we are trying to use different fluorescence spectra to detect biological molecules commonly found in cancerous colorectal tissue and thereby differentiate the cancerous and non-cancerous colorectal polyps more accurately and specifically. In this paper, we use Fluorescence Lifetime Spectrometer (Edinburgh Instruments FL920) to measure decay time of autofluorescence of colorectal cancerous and normal tissue sample. All specimens are from Department of Colorectal Surgery, Singapore General Hospital. The tissues are placed in the time-resolved autofluorescence instrument, which records and calculates the decay time of the autofluorescence in the tissue sample at the excitation and emission wavelengths pre-determined from a conventional spectrometer. By studying the decay time,τ, etc. for cancerous and normal tissue, we aim to present time-resolved autofluorescence as a feasible technique for earlier detection of malignant colorectal tissues. By using this concept, we try to contribute an algorithm even an application tool for real time early diagnosis of colorectal cancer for clinical services.

Paper Details

Date Published: 14 April 2006
PDF: 10 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910M (14 April 2006); doi: 10.1117/12.663257
Show Author Affiliations
Sheng Fu M.D., Nanyang Technological Univ. (Singapore)
Leong-Chuan Kwek, Nanyang Technological Univ. (Singapore)
Teck-Chee Chia, Nanyang Technological Univ. (Singapore)
Chu-Sing Lim, Nanyang Technological Univ. (Singapore)
Choong-Leong Tang, Singapore General Hospital (Singapore)
Wuan-Suan Ang, Nanyang Technological Univ. (Singapore)
Miao-Chang Zhou, Nanyang Technological Univ. (Singapore)
Po-Ling Loke, Nanyang Technological Univ. (Singapore)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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