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Proceedings Paper

Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics
Author(s): Pierre Marquet; Benjamin Rappaz; Tristan Colomb; Florian Charrière; Jonas Kühn; Yves Emery; Etienne Cuche; Christian Depeursinge; Pierre Magistretti
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Paper Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

Paper Details

Date Published: 14 April 2006
PDF: 5 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910U (14 April 2006); doi: 10.1117/12.662988
Show Author Affiliations
Pierre Marquet, Ctr. de Neurosciences Psychiatriques (Switzerland)
Benjamin Rappaz, École Polytechnique Fédérale de Lausanne (Switzerland)
Tristan Colomb, École Polytechnique Fédérale de Lausanne (Switzerland)
Florian Charrière, École Polytechnique Fédérale de Lausanne (Switzerland)
Jonas Kühn, École Polytechnique Fédérale de Lausanne (Switzerland)
Yves Emery, Lyncée Tec SA (Switzerland)
Etienne Cuche, Lyncée Tec SA (Switzerland)
Christian Depeursinge, École Polytechnique Fédérale de Lausanne (Switzerland)
Pierre Magistretti, Ctr. de Neurosciences Psychiatriques (Switzerland)
École Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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