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Proceedings Paper

Effect of 655 nm laser different powers on dog sperm motility parameters
Author(s): M. I. Corral-Baqués; T. Rigau; M. M. Rivera; J. E. Rodríguez-Gil; J. Rigau
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Paper Abstract

Introduction: One of the most appreciated features of the sperm is its motility, which depends on a big energy consumption despite differences among species. Laser acts direct or indirectly on mitochondria increasing ATP production. Material and method: By means of a Computer Aided Sperm Analysis (CASA) we have studied the effects of a 655 nm continuous wave diode laser irradiation at different power outputs with a dose of 3.3418 J on sperm motility. After an eosine-nigrosine stain to establish its quality, the second fraction of fresh beagle dog sperm was divided into 5 groups, 1 control and four to be irradiated respectively with an average output power of 6.84 mW, 15.43 mW, 33.05 mW and 49.66 mW. At times 0 and 45 minutes from irradiation pictures were taken and analysed with the Sperm class Analyzer SCA2002 programme. The motility parameters of 4987 spermatozoa studied were: curvilinear velocity (VCL), progressive velocity (VSL), straightness (STR), wobble (WOB), average path velocity (VAP), linearity (LIN), mean amplitude of lateral head displacement (ALHmed), beat cross frequency (BCF) and the total motility (MT). At time 15 minutes after irradiation a hypoosmotic swelling test (HOST) was done. Results: Several motility parameters that affect the overall motile sperm subpopulation structure have been changed by different output powers of a 655 nm diode laser irradiation, and prevents the decrease of the sperm motility properties along time.

Paper Details

Date Published: 14 April 2006
PDF: 9 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 619104 (14 April 2006); doi: 10.1117/12.662585
Show Author Affiliations
M. I. Corral-Baqués, Rovira i Virgili Univ. (Spain)
T. Rigau, Autonomous Univ. of Barcelona (Spain)
M. M. Rivera, Autonomous Univ. of Barcelona (Spain)
J. E. Rodríguez-Gil, Autonomous Univ. of Barcelona (Spain)
J. Rigau, Rovira i Virgili Univ. (Spain)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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