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Proceedings Paper

Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates
Author(s): Jacques Derouard; Antoine Delon; Rodolphe Jaffiol; Cyrille Vézy
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Paper Abstract

Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure the areal absolute concentration of fluorophores deposited on a substrate or imbedded in a thin film, with a resolution of a few micrometers. The principle is to translate the solid substrate in front of a confocal fluorescence microscope objective and to record the subsequent fluctuations of the fluorescence intensity. The validity of this concept is investigated on model substrates (fluorescent microspheres), DNA-chips, and dye-stained histidine molecules anchored on silanized glass surfaces.

Paper Details

Date Published: 14 April 2006
PDF: 8 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910C (14 April 2006); doi: 10.1117/12.662294
Show Author Affiliations
Jacques Derouard, Lab. de Spectrométrie Physique, CNRS, Univ. Joseph Fourier Grenoble I (France)
Antoine Delon, Lab. de Spectrométrie Physique, CNRS, Univ. Joseph Fourier Grenoble I (France)
Rodolphe Jaffiol, Lab. de Spectrométrie Physique, CNRS, Univ. Joseph Fourier Grenoble I (France)
Univ. de Technologie de Troyes (France)
Cyrille Vézy, Lab. de Spectrométrie Physique, CNRS, Univ. Joseph Fourier Grenoble I (France)


Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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