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Proceedings Paper

Study of polycation effects on erythrocyte agglutination mediated by anti-glycophorins using microscopic image digital analysis
Author(s): B. Riquelme; D. Dumas; F. Relancio; A. Fontana; A. Alessi; P. Foresto; C. Grandfils; J. Stoltz; J. Valverde
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Paper Abstract

The aim of this work was to study synthetic polycation effects on erythrocyte agglutination mediated by anti-glycophorin using image digital analysis. Polycations are oligomers or polymers of natural or synthetic origin, which bear a great number of positive charges at pH 7.4. Several of these polycations are nowadays used in clinic for human and veterinary purposes. New applications of polycations to the development of new drug delivery systems are investigated, in order to promote the drug absorption through the gastro-intestinal and blood brain barriers. However, up to now, there are no clear relationships between macromolecular features of polycations (molecular weight, mean charge density, charge repartition, etc.) and their interactions with blood elements (which bear superficial negative charges). The interaction on the red blood cell membrane with synthetic polycations having well-controlled macromolecular features and functionalized with pendent polyethylene glycol segments was investigated. The alterations over stationary and dynamic viscoelastic properties of erythrocyte membranes were analyzed through laser diffractometry. Image digital analysis was used to study erythrocyte agglutination mediated by anti-glycophorin. Results show different reactivities of the polycations on the erythrocyte membrane. These findings could provide more information about the mechanisms of polycation interaction on erythrocyte membranes. We consider that this work could provide useful tools to understand and improve the haemocompatibility of polycations and enlarge their potential in clinic.

Paper Details

Date Published: 14 April 2006
PDF: 10 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61911G (14 April 2006); doi: 10.1117/12.662141
Show Author Affiliations
B. Riquelme, Insituto de Física Rosario (Argentina)
Univ. Nacional de Rosario (Argentina)
D. Dumas, Univ. Henry Poincaré (France)
F. Relancio, Insituto de Física Rosario (Argentina)
A. Fontana, Univ. Nacional de Rosario (Argentina)
A. Alessi, Univ. Nacional de Rosario (Argentina)
P. Foresto, Instituto de Física Rosario (Argentina)
Univ. Nacional de Rosario (Argentina)
C. Grandfils, Univ. de Liège (Belgium)
J. Stoltz, Univ. Henry Poincaré (France)
J. Valverde, Instituto de Física Rosario (Argentina)
Univ. Nacional de Rosario (Argentina)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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