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Proceedings Paper

Total internal reflection energy transfer (TIRET) microscopy for analysis of focal adhesions in living cells
Author(s): Brigitte Angres; Heiko Steuer; Michael Wagner; Petra Weber; Herbert Schneckenburger
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Paper Abstract

Total internal reflection fluorescence microscopy (TIRFM) is used to measure non-radiative energy transfer between membrane associated proteins in living cells. Measurements are concentrated on focal contacts and their associated proteins focal adhesion kinase (FAK) and Paxillin (Pax) which play major roles with respect to cell migration, growth, and survival. These proteins are visualized after fusion with variants of green fluorescent protein (ECFP and EYFP), and an intermolecular energy transfer ECFP -> EYFP is deduced from fluorescence spectra as well as from fluorescence decay kinetics of single cells.

Paper Details

Date Published: 14 April 2006
PDF: 7 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910A (14 April 2006); doi: 10.1117/12.662073
Show Author Affiliations
Brigitte Angres, Univ. Tübingen (Germany)
Heiko Steuer, Univ. Tübingen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Petra Weber, Hochschule Aalen (Germany)
Herbert Schneckenburger, Hochschule Aalen (Germany)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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