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Proceedings Paper

Quantum dots as resonance energy transfer acceptors for monitoring biological interactions
Author(s): Niko Hildebrandt; Loïc Charbonnière; Raymond F. Ziessel; Hans-Gerd Löhmannsröben
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Paper Abstract

Due to their extraordinary photophysical properties CdSe/ZnS core/shell nanocrystals (quantum dots) are excellent luminescence dyes for fluorescence resonance energy transfer (FRET) systems. By using a supramolecular lanthanide complex with central terbium cation as energy donor, we show that commercially available biocompatible biotinilated quantum dots are excellent energy acceptors in a time-resolved FRET fluoroimmunoassay (FRET-FIA) using streptavidin-biotin binding as biological recognition process. The efficient energy transfer is demonstrated by quantum dot emission sensitization and a thousandfold increase of the nanocrystal luminescence decay time. A Foerster Radius of 90 Å and a picomolar detection limit were achieved in quantum dot borate buffer. Regarding biological applications the influence of bovine serum albumin (BSA) and sodium azide (a frequently used preservative) to the luminescence behaviour of our FRET-system is reported.

Paper Details

Date Published: 14 April 2006
PDF: 9 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910W (14 April 2006); doi: 10.1117/12.660660
Show Author Affiliations
Niko Hildebrandt, Univ. Potsdam (Germany)
Loïc Charbonnière, Lab. de Chimie Moléculaire (France)
Raymond F. Ziessel, Lab. de Chimie Moléculaire (France)
Hans-Gerd Löhmannsröben, Univ. Potsdam (Germany)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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