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Proceedings Paper

Protease-activated quantum dot probes
Author(s): Emmanuel Chang; Jiantang Sun; Jordan S. Miller; William W. Yu; Vicki L. Colvin; Jennifer L. West; Rebekah Drezek
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Paper Abstract

We demonstrate a novel quantum dot based probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This probe may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically-degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Peptide cleavage results in release of AuNPs and restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 hours of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

Paper Details

Date Published: 14 April 2006
PDF: 10 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61911E (14 April 2006); doi: 10.1117/12.660485
Show Author Affiliations
Emmanuel Chang, Rice Univ. (United States)
Jiantang Sun, Rice Univ. (United States)
Jordan S. Miller, Rice Univ. (United States)
William W. Yu, Rice Univ. (United States)
Vicki L. Colvin, Rice Univ. (United States)
Jennifer L. West, Rice Univ. (United States)
Rebekah Drezek, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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