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Proceedings Paper

Cell viability test after laser guidance
Author(s): Tabitha N. Rosenbalm; Sarah Owens; Daniel Bakken; Bruce Z. Gao
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Paper Abstract

To precisely control the position of multiple types of cells in a coculture for the study of cell-cell interactions, we have developed a laser micropatterning technique. The technique employs the optical forces generated by a weakly focused laser beam. In the beam's focal region, the optical force draws microparticles, such as cells, into the center of the beam, propels them along the beam axis, and guides them onto a target surface. Specific patterns are created through computercontrolled micromanipulation of the substrate relative to the laser beam. Preliminary data have demonstrated cell viability after laser guidance. This project was designed to systematically vary the controllable laser parameters, namely, intensity and exposure time of the laser on single cells, and thus determine the laser parameters that allow negligible cell damage with functional cellular position control. To accomplish this goal, embryonic day 7 (E7) chick forebrain neurons were cultured in 35 mm petri dishes. Control and test cells were selected one hour after cell placement to allow cell attachment. Test cells were subjected to the laser at the focal region. The experimental parameters were chosen as: wavelength - 800 nm, intensities - 100 mW, 200 mW, and 300 mW, and exposure times - 10 s and 60 s. Results were analyzed based on neurite outgrowth and the Live/Dead assay (Viability/Cytoxicity kit from Molecular Probes). No statistical difference (p >> 0.1, student t-test) in viability or function was found between the control neurons and those exposed to the laser. This confirms that laser guidance seems to be a promising method for cellular manipulation.

Paper Details

Date Published: 28 February 2006
PDF: 8 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 608418 (28 February 2006); doi: 10.1117/12.651467
Show Author Affiliations
Tabitha N. Rosenbalm, Clemson Univ. (United States)
Sarah Owens, Louisiana State Univ. (United States)
Daniel Bakken, Clemson Univ. (United States)
Bruce Z. Gao, Clemson Univ. (United States)

Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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