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Proceedings Paper

Detection of 2-photon oxidation from a NIR laser using confocal microscopy
Author(s): Kurt J. Schuster; Larry E. Estlack; Benjamin A. Rockwell; Michael L. Denton
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Paper Abstract

Recent studies have determined that photochemical oxidation in cultured cells can be detected at peak irradiances as low as 8.5x108 W cm-2 (87-fs pulse). Fluorescent dyes, such as CM-H2DCFDA, enable us to quantify the oxidation response of cells to mode-locked near-infrared (NIR) laser exposure. Using a modified confocal microscope, we characterize the time-dependent 2-photon induced fluorescence generated from a given NIR laser exposure. When cultured cells were then pre-loaded with antioxidants, ascorbic acid or N-acetyl-L-cysteine (NAC), they inhibit nonlinear oxidation with different efficiencies, providing insight regarding mechanisms of damage.

Paper Details

Date Published: 28 February 2006
PDF: 9 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60841H (28 February 2006); doi: 10.1117/12.644651
Show Author Affiliations
Kurt J. Schuster, Northrop Grumman Corp. (United States)
Larry E. Estlack, Conceptual Mindworks, Inc. (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)
Michael L. Denton, Northrop Grumman Corp. (United States)

Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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