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Proceedings Paper

Wavelet-based synchronization of nongated confocal microscopy data for 4D imaging of the embryonic heart
Author(s): Michael Liebling; Arian S. Forouhar; Morteza Gharib; Scott E. Fraser; Mary E. Dickinson
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Paper Abstract

With the availability of new confocal laser scanning microscopes, fast biological processes, such as the blood flow in living organisms at early stages of the embryonic development, can be observed with unprecedented time resolution. When the object under study has a periodic motion, e.g. a beating embryonic heart, the imaging capabilities can be extended to retrieve 4D data. We acquire nongated slice-sequences at increasing depth and retrospectively synchronize them to build dynamic 3D volumes. Here, we present a synchronization procedure based on the temporal correlation of wavelet features. The method is designed to handle large data sets and to minimize the influence of artifacts that are frequent in fluorescence imaging techniques such as bleaching, nonuniform contrast, and photon-related noise.

Paper Details

Date Published: 17 September 2005
PDF: 6 pages
Proc. SPIE 5914, Wavelets XI, 591409 (17 September 2005); doi: 10.1117/12.615232
Show Author Affiliations
Michael Liebling, California Institute of Technology (United States)
Arian S. Forouhar, California Institute of Technology (United States)
Morteza Gharib, California Institute of Technology (United States)
Scott E. Fraser, California Institute of Technology (United States)
Mary E. Dickinson, California Institute of Technology (United States)
Baylor College of Medicine (United States)

Published in SPIE Proceedings Vol. 5914:
Wavelets XI
Manos Papadakis; Andrew F. Laine; Michael A. Unser, Editor(s)

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