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Proceedings Paper

A fluorescence methodology for assessing the polarity and composition of novel thermoresponsive hydrophylic/hydrophobic copolymer system
Author(s): Boguslaw Szczupak; Alan G. Ryder; Yuri A. Rochev; Andrey S. Klymchenko; Alexander Gorelov; Thomas J. Glynn
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Paper Abstract

The use of designed polymer coatings for specific applications such as drug delivery or modifying cell response is a critical aspect of medical device manufacturing. The chemical composition and physical characteristics of thin polymer coatings need to be analysed in-situ and this can present difficulties for traditional analytical methods. For example, changes in the polarity of polymer coatings are typically measured using the contact angle (CA) method. This is a simple process and gives good results however; it cannot be used to measure very hydrophilic polymers, or to analyse features smaller than a couple of mm in size. There is a need for a non-contact method for polarity measurement that is suitable for hydrophilic polymers on a macro- and microscopic scale. 4'-diethylamino-3-hydroxyflavone (FE), 5, 6-benzo-4'-diethylamino-3-hydroxyflavone (BFE), and 4'-diethylamino-3-hydroxy-7-methoxyflavone (MFE) are fluorescence probes based on 3-hydroxyflavone. They respond to environment perturbations by shift and changes in the relative intensity of two well-separated bands in the emission spectra. These bands originate from an excited state intramolecular proton transfer (ESIPT) reaction. We have incorporated FE, BFE, and MFE into a novel thermoresponsive hydrophilic/hydrophobic copolymer system (NIPAM-NtBA) and studied its fluorescence behaviour. The fluorescence emission spectra depend strongly on copolymer composition, with increasing hydrophobicity (greater NtBA fraction) leading to a decrease in the value of log (IN*/IT*). This allows for the non-contact, measurement of the exact composition and surface energy of the copolymer system.

Paper Details

Date Published: 3 June 2005
PDF: 11 pages
Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); doi: 10.1117/12.605117
Show Author Affiliations
Boguslaw Szczupak, National Univ. of Ireland/Galway (Ireland)
Alan G. Ryder, National Univ. of Ireland/Galway (Ireland)
Yuri A. Rochev, National Univ. of Ireland/Galway (Ireland)
Andrey S. Klymchenko, Univ. Louis Pasteur (France)
Alexander Gorelov, Univ. College Dublin (Ireland)
Thomas J. Glynn, National Univ. of Ireland/Galway (Ireland)

Published in SPIE Proceedings Vol. 5826:
Opto-Ireland 2005: Optical Sensing and Spectroscopy
Gerard D. O'Sullivan; Brian D. MacCraith; Hugh James Byrne; Enda McGlynn; Alan G. Ryder, Editor(s)

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