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Proceedings Paper

A dual-detector based optical fluorescence imaging system
Author(s): Jason Y. Chen; Yuhua Li; Hong Liu
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Paper Abstract

A dual-CCD based optical fluorescence system has been developed to facilitate ratio imaging. The system consists of two custom cameras capable of operating at 490 frames per second, with 128 by 128 detector elements, and 12 bits digitization. Tissue-simulating gel phantoms containing 5-(and-6)-carboxy seminaphthofluoresceins-1 (SNAFL-1) fluorescent dye were constructed. Using an excitation light at a wavelength of 489±11 nm and appropriate filtering, the fluorescence intensities of the dye-containing gel phantoms at different emission wavelengths were measured by the system. The dual-detector system can simultaneously collect two different intensity peaks. Gel phantoms with dye concentrations ranging from 0.00001 mg/mL to 0.1 mg/mL were used. Our results indicate that the dual-CCD fluorescence system is capable of detecting fluorescence signals at very low dye concentration (as low as 0.0001 mg/mL). To simulating real clinical situations, dye-containing gel was placed under normal gel of different thickness. Our results showed that the system can capture significant fluorescence signals of dye-containing gel under normal gel of a thickness up to 0.73 cm. The intensity ratios between different emission peaks were also measured. The system is a useful research tool in some biomedical experiments.

Paper Details

Date Published: 29 March 2005
PDF: 7 pages
Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); doi: 10.1117/12.593801
Show Author Affiliations
Jason Y. Chen, Univ. of Oklahoma (United States)
Yuhua Li, Univ. of Oklahoma (United States)
Hong Liu, Univ. of Oklahoma (United States)

Published in SPIE Proceedings Vol. 5696:
Complex Dynamics and Fluctuations in Biomedical Photonics II
Valery V. Tuchin, Editor(s)

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