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Proceedings Paper

Herbal tea extract combined with light-induced significant in vitro cytotoxicity of human bladder cancer cells
Author(s): Unyime Nseyo M.D.; Albert Kim; Nicholas E. Stavropoulos M.D.; Dimitris Skalkos; U. U. Nseyo; Theodore D. Chung M.D.
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Paper Abstract

The anti-inflammatory, anti-microbial, antiviral, and antidepressant activities of the Greek herb, Hypericum Perforatum L, HP L, have been attributed to the total extract or single constituents. We investigated the use of the extract,specifically of the polar methanolic fraction (PMF) of Epirus’HPL in photodynamic therapy (PDT) alone and in combination with recombinant Interferon-a2b (IFN) and gemcitabine (GCB) in the treatment of human bladder cancer cells. The PMF was extracted from the dry herb with methanol, followed by liquid-liquid extraction with petroleum ether. T-24 bladder cancer cells were plated (105 cells/well) and placed in the incubator (370 C, 5%CO) for 24 hours prior to addition of drugs. PMF 60ug/ml was added and incubation continued. After 24 hours, the cells were subjected to laser light (630nm) treatment with 0, 1, 4 and 8 Joules. After reincubation for 24 hours, IFN, (50,000 IU) or GCB, (2ug/ml) was added to the PDT-treated cells. After this incubation cell survival was assessed by the MTT assay. PMF-PDT alone-induced percent cell kill of 0%, 8%, 44% and 80% versus 31%, 64 and 86 % for PMF-PDT and IFN, versus 63%, 80% and 88% for MPF-PDT plus GCB at 1, 2, 4 and 8 Joules respectively. IFN and GCB induced 20% and 53% cell kill respectively. Our data suggest that MPF may be an effective agent for in vitro photodynamic therapy. PMF-PDT combined with Intron A, or gemcitabine achieved improved kill of cultured bladder cancer cells. Confirmation of these results in preclinical studies may lead to clinical trials.

Paper Details

Date Published: 25 April 2005
PDF: 8 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.592563
Show Author Affiliations
Unyime Nseyo M.D., Virginia Commonwealth Univ. (United States)
McGuire VA Medical Ctr. (United States)
Albert Kim, Virginia Commonwealth Univ. (United States)
Nicholas E. Stavropoulos M.D., Hatzikosta General Hospital (Greece)
Dimitris Skalkos, Univ. of Ioannina (Greece)
U. U. Nseyo, Virginia Commonwealth Univ. (United States)
Harvard Univ. (United States)
Theodore D. Chung M.D., Virginia Commonwealth Univ. (United States)

Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Kenton W. Gregory M.D.; Abraham Katzir; Nikiforos Kollias; Reza S. Malek M.D.; Henry Hirschberg M.D.; Kenneth Eugene Bartels D.V.M.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Lawrence S. Bass M.D.; Werner T. W. de Riese; Karen M. McNally-Heintzelman, Editor(s)

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