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Proceedings Paper

In vivo multiphoton tomography of skin as a tool to study the effects of topically applied probes and UV exposure
Author(s): Iris Riemann; Alexander Ehlers; Annette Reif; Jens Kobow; Karsten Konig
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Paper Abstract

High resolution optical tomography studies on topically applied probes and UVA exposed skin were performed with the novel imaging system DermaInspect. 3D images with submicron resolution based on the two-photon excitation of the probes and the autofluorescence of epidermal cells and structures with femtosecond laser pulses in the near infrared (NIR) spectral range between 750 nm and 850 nm were obtained. The distribution of natural endogenous fluorophores, mainly keratin and NAD(P)H, as well as of the exogenous probe molecules was detected in situ. The high resolution imaging system DermaInspect offers an unique chance of in situ, non-invasive drug screening in skin on a molecular level and to study the effect of UV exposure.

Paper Details

Date Published: 25 April 2005
PDF: 6 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.590817
Show Author Affiliations
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Alexander Ehlers, Fraunhofer Institute of Biomedical Technology (Germany)
Annette Reif, Univ. Jena (Germany)
Jens Kobow, JenLab GmbH (Germany)
Karsten Konig, Fraunhofer Institute of Biomedical Technology (Germany)

Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Kenton W. Gregory M.D.; Abraham Katzir; Nikiforos Kollias; Reza S. Malek M.D.; Henry Hirschberg M.D.; Kenneth Eugene Bartels D.V.M.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Lawrence S. Bass M.D.; Werner T. W. de Riese; Karen M. McNally-Heintzelman, Editor(s)

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