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Proceedings Paper

Optical and acoustical monitoring of femtosecond laser-induced intracellular contrast agents: initial cell culture studies
Author(s): Marwa J. Zohdy; Christine Tse; Jing Yong Ye; Lajos P. Balogh; Theodore B. Norris; Matthew O'Donnell
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Paper Abstract

Acoustical monitoring of laser-induced optical breakdown can be used as an important tool for diagnostics and therapeutics in living cells. Laser-induced intracellular microbubbles provide measurable contrast when detected with high-frequency ultrasound, and the bioeffects of these bubbles can be controlled to be within two distinct regimes. In the nondestructive regime, a single, transient, detectable bubble can be generated within a cell, without affecting its viability. In the destructive regime, the induced photodisruption can kill a target cell. To generate and monitor this range of effects in real time, we have developed a system integrating a femtosecond pulsed laser source with optical and acoustical microscopy. Experiments were performed on monolayers of Chinese hamster ovary cells. A Ti:Sapphire laser (793 nm wavelength, 100 fs pulse duration) was pulsed at 3.8 kHz and tightly focused to a 1 μm spot within each cell, and a high-frequency (50 MHz) ultrasonic transducer monitored the generated bubble with continuous pulse-echo recordings. The photodisruption was also observed with bright field optical microscopy, and cell viability was assessed after laser exposure using a colorimetric live/dead stain. By controlling laser pulse fluence, exposure duration, and the intracellular location of the laser focus, either nondestructive or destructive bubbles could be generated.

Paper Details

Date Published: 25 April 2005
PDF: 10 pages
Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); doi: 10.1117/12.589687
Show Author Affiliations
Marwa J. Zohdy, Univ. of Michigan (United States)
Christine Tse, Univ. of Michigan (United States)
Jing Yong Ye, Univ. of Michigan (United States)
Lajos P. Balogh, Univ. of Michigan (United States)
Theodore B. Norris, Univ. of Michigan (United States)
Matthew O'Donnell, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 5697:
Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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