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Proceedings Paper

Addition of ionizing radiation or hyperthermia enhances PDT efficacy in glioma spheroids
Author(s): Steen J. Madsen; Chung-Ho Sun; Bruce J. Tromberg; Josephine Ni; Henry Hirschberg
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Paper Abstract

The effects of 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and gamma radiation, and PDT and hyperthermia (HT) are investigated in human glioma spheroids. In the case of ionizing radiation and PDT, the degree of interaction between the two modalities depends in a complex manner on a number of factors, including light fluence, fluence rate and radiation dose. It is shown that, under certain conditions, PDT and gamma radiation interact synergistically to produce enhanced cell kill. The degree of interaction appears to be independent of both sequence and time intervals investigated. TUNEL assays show that low fluence-rate ALA-PDT is a very efficient inducer of apoptosis, whereas neither high-fluence rate PDT nor ionizing radiation results in significant apoptosis in this system. Synergistic interactions between HT and PDT are observed when the two modalities are given concurrently. The degree of synergism increases with increasing temperature and light fluence. It is shown that apoptosis is the primary mode of cell death following combined HT and low-fluence rate ALA-PDT.

Paper Details

Date Published: 25 April 2005
PDF: 12 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.589669
Show Author Affiliations
Steen J. Madsen, Univ. of Nevada/Las Vegas (United States)
Chung-Ho Sun, Beckman Laser Institute, Univ. of California/Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute, Univ. of California/Irvine (United States)
Josephine Ni, Beckman Laser Institute, Univ. of California/Irvine (United States)
Henry Hirschberg, Rikshospitalet (Norway)
Beckman Laser Institute, Univ. of California/Irvine (United States)

Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Kenton W. Gregory M.D.; Abraham Katzir; Nikiforos Kollias; Reza S. Malek M.D.; Henry Hirschberg M.D.; Kenneth Eugene Bartels D.V.M.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Lawrence S. Bass M.D.; Werner T. W. de Riese; Karen M. McNally-Heintzelman, Editor(s)

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