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Proceedings Paper

Photoacoustic detection of neovascularities in skin graft
Author(s): Mutsuo Yamazaki; Shunichi Sato; Daizo Saitoh; Miya Ishihara; Yoshiaki Okada; Hiroshi Ashida; Minoru Obara
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Paper Abstract

We previously proposed a new method for monitoring adhesion of skin graft by measuring photoacoustic (PA) signal originated from the neovascularities. In this study, immunohistochemical staining (IHC) with CD31 antibody was performed for grafted skin tissue to observe neovascularity, and the results were compared with PA signals. We also used a laser Doppler imaging (LDI) to observe blood flow in the grafted skin, and sensitivity of PA measurement and that of LDI were compared. In rat autograft models, PA signals were measured for the grafted skin at postgrafting times of 0-48 h. At 6 h postgrafting, PA signal was observed in the skin depth region of 500-600 mm, while the results of IHC showed that angiogenesis occurred at the depth of about 600 mm. Depths at which PA signal and angiogenesis were observed decreased with postgrafting time. These indicate that the PA signal observed at 6 h postgrafting originated from the neovascularities in the skin graft. Results of LDI showed no blood-originated signal before 48 h postgrafting. These findings suggest that PA measurement is effective in monitoring the adhesion of skin graft in early stage after transplantation.

Paper Details

Date Published: 25 April 2005
PDF: 4 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.588879
Show Author Affiliations
Mutsuo Yamazaki, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Daizo Saitoh, National Defense Medical College (Japan)
Miya Ishihara, National Defense Medical College (Japan)
Yoshiaki Okada, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong M.D.; Eugene A. Trowers M.D.; Kenton W. Gregory M.D.; Abraham Katzir; Nikiforos Kollias; Reza S. Malek M.D.; Henry Hirschberg M.D.; Kenneth Eugene Bartels D.V.M.; Steen J. Madsen; Lloyd P. Tate V.D.M.; Lawrence S. Bass M.D.; Werner T. W. de Riese; Karen M. McNally-Heintzelman, Editor(s)

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