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Proceedings Paper

Microlymphatic flow using fast video microscopy
Author(s): J. Brandon Dixon; David C. Zawieja; Anatoly A. Gashev M.D.; Gerard L. Cote
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Paper Abstract

Despite advances in the measurement of lymphatic flow, little is known about the actual velocities of flow in microlymphatic (~100 um diameter) vessels. In this paper, video microscopy and particle tracking methods were adapted and integrated with an ultra high-speed imaging camera to obtain measurements of high-speed lymph velocities that previous systems were incapable of measuring. In this study, a mesenteric microlymphatic vessel in a loop of the small intestine of a male Sprague-Dawley rat was exteriorized and imaged at a rate of 500 frames per second (fps) for several contraction sequences. Lymph velocity was shown to fluctuate cyclically with the vessel wall contractions and ranged from -1 to 4 mm/sec through a ten second sequence.

Paper Details

Date Published: 24 March 2005
PDF: 4 pages
Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); doi: 10.1117/12.588633
Show Author Affiliations
J. Brandon Dixon, Texas A&M Univ. (United States)
David C. Zawieja, Texas A&M Health Science Ctr. (United States)
Anatoly A. Gashev M.D., Texas A&M Health Science Ctr. (United States)
Gerard L. Cote, Texas A&M Univ. (United States)


Published in SPIE Proceedings Vol. 5701:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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