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Proceedings Paper

Generalized temporal sampling with active illumination in optical microscopy
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Paper Abstract

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overlapping inner-products and because only positive signals (intensities) can be measured by cameras. We present a method to collect videos of monochromatic objects by projecting the incoming signal at each pixel in a temporal B-spline space of degree 0, 1, or 2 by using a conventional RGB camera and a modulated three-color light source for illumination. Specifically, we solve the basis function overlap problem by multiplexing the acquisition in different color ranges and use B-spline pieces (which are positive) as projection kernels of a biorthogonal projection-expansion bases pair. The steps to recover signal samples include spectral unmixing and inverse filtering. Reconstructions we obtained from simulated and experimentally-acquired microscopy data demonstrate the feasibility of our approach.

Paper Details

Date Published: 9 September 2019
PDF: 9 pages
Proc. SPIE 11138, Wavelets and Sparsity XVIII, 1113816 (9 September 2019); doi: 10.1117/12.2529526
Show Author Affiliations
Christian Jaques, Idiap Research Institute (Switzerland)
École Polytechnique Fédérale de Lausanne (Switzerland)
Michael Liebling, Idiap Research Institute (Switzerland)
Univ. of California, Santa Barbara (United States)

Published in SPIE Proceedings Vol. 11138:
Wavelets and Sparsity XVIII
Dimitri Van De Ville; Manos Papadakis; Yue M. Lu, Editor(s)

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