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Proceedings Paper

Tumour detection and staging through multimodal fibre-probe spectroscopy
Author(s): Enrico Baria; Suresh Anand; Riccardo Fantechi; Simone Morselli; Mauro Gacci; Marco Carini; Riccardo Cicchi; Francesco S. Pavone
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Paper Abstract

Urothelial carcinoma (UC) is the most common type of bladder cancer. The gold standard for detecting UC is white-light cystoscopy, which is followed by tissue biopsy and pathological examination. However, such process is invasive, timeconsuming and prone to sampling errors. In this framework, optical spectroscopy techniques provide fast, label-free and non-invasive alternatives to standard histopathology. Thus, the aim of this study is to discriminate normal bladder tissues from urothelial tumours, and to identify the different stages of the disease, by means of combined auto-fluorescence, diffuse reflectance and Raman spectroscopy. In fact, these techniques were implemented in a compact and transportable setup based on two optical fibre probes: one coupled to fluorescence and reflectance excitation sources, while the other one to the 785 nm laser. Raman, fluorescence and reflected light signals were collected though the same probe used for excitation and sent to a spectrograph. We used this experimental setup for studying fresh biopsies of urothelial tumour and healthy bladder collected from 32 patients undergoing Transurethral Resection of Bladder Tumours (TURBT). Scoring methods based on ratiometric approach and Principal Component Analysis (PCA) allowed not only to discriminate healthy biopsies from tumour ones, but also to recognize three tumour stages.

Paper Details

Date Published: 17 May 2018
PDF: 5 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106851X (17 May 2018); doi: 10.1117/12.2317996
Show Author Affiliations
Enrico Baria, National Institute of Optics (Italy)
Suresh Anand, National Institute of Optics (Italy)
Riccardo Fantechi, Univ. degli Studi di Firenze (Italy)
Simone Morselli, Univ. degli Studi di Firenze (Italy)
Mauro Gacci, Univ. degli Studi di Firenze (Italy)
Marco Carini, Univ. degli Studi di Firenze (Italy)
Riccardo Cicchi, National Institute of Optics (Italy)
Univ. degli Studi di Firenze (Italy)
Francesco S. Pavone, National Institute of Optics (Italy)
LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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