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Proceedings Paper

Label-free imaging of lipid droplets in cells by stimulated Raman microscopy
Author(s): Maria Antonietta Ferrara; Angela Filograna; Annalisa D'Arco; Rajeev Ranjan; Michele Larobina; Carmen Valente; Luigi Sirleto
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Paper Abstract

Recent technological developments in ultrafast laser physics have permitted to make new kind of nonlinear microscopies, as microscopy based on Stimulated Raman scattering. These techniques are based on vibrational contrast mechanism for imaging with high sensitivity, high spatial and spectral resolution and 3D sectioning capability. The interest in the study of lipids and the possibility to image lipid droplets, thanks to their isolated Raman peaks associated with vibrational C-H bond, have encouraged investigation and identification of lipid structures inside cells, taking advantage of Stimulated Raman Scattering (SRS) imaging. In this work, we report and discuss label free images on biological environmental and structural analysis, to detect lipid microstructures inside adipocyte cells.

Paper Details

Date Published: 24 May 2018
PDF: 8 pages
Proc. SPIE 10677, Unconventional Optical Imaging, 106770P (24 May 2018); doi: 10.1117/12.2317993
Show Author Affiliations
Maria Antonietta Ferrara, Istituto per la Microelettronica e Microsistemi (Italy)
Angela Filograna, Istituto di Biochimica delle Proteine (Italy)
Annalisa D'Arco, Istituto per la Microelettronica e Microsistemi (Italy)
Rajeev Ranjan, Istituto per la Microelettronica e Microsistemi (Italy)
Michele Larobina, Consiglio Nazionale delle Ricerche (Italy)
Carmen Valente, Istituto di Biochimica delle Proteine (Italy)
Luigi Sirleto, Istituto per la Microelettronica e Microsistemi (Italy)

Published in SPIE Proceedings Vol. 10677:
Unconventional Optical Imaging
Corinne Fournier; Marc P. Georges; Gabriel Popescu, Editor(s)

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