Share Email Print

Proceedings Paper

Quantitative phase imaging in turbid media by coherence controlled holographic microscopy
Author(s): Radim Chmelik; Miroslav Duris; Lenka Strbkova
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Quantitative phase imaging (QPI) became an important technique for label-free biomedical imaging suitable particularly for observation of live cell and dry-mass profiling. Extension of this technique to objects immersed in turbid medium is highly desirable with respect to the need of non-invasive observation of live cells in real 3D environments. Coherencecontrolled holographic microscopy is capable of QPI through turbid media owing to coherence gating induced in transmitted-light geometry by low spatial coherence of illumination. Using this approach, QPI of object in turbid medium can be formed both by ballistic and multiply scattered photons. Moreover, the particular QPIs formed by ballistic and scattered photons can be superimposed thus yielding synthetic QPI of substantially improved image quality. We support the theoretical reasoning of the effect by experimental data.

Paper Details

Date Published: 24 May 2018
PDF: 11 pages
Proc. SPIE 10677, Unconventional Optical Imaging, 106771C (24 May 2018); doi: 10.1117/12.2309907
Show Author Affiliations
Radim Chmelik, CEITEC Brno Univ. of Technology (Czech Republic)
Miroslav Duris, Brno Univ. of Technology (Czech Republic)
Lenka Strbkova, CEITEC Brno Univ. of Technology (Czech Republic)

Published in SPIE Proceedings Vol. 10677:
Unconventional Optical Imaging
Corinne Fournier; Marc P. Georges; Gabriel Popescu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?