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Proceedings Paper

Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin
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Paper Abstract

In this study, the optical properties of glycated (HbA1c) and non-glycated (Hb) hemoglobin are compared using refractometry, fluorescence and Raman spectroscopy. The fluorescence measured at an excitation wavelength of 270 nm indicates differences in the molecular structure of hemoglobins. Analysis of the spectral shift of Raman spectra also showed variations indicating differences in their molecular structure. The refractive index measured in the visible and near IR regions for different temperatures allowed for quantification of mean values of temperature increment, which are quite different as dn/dT= –(1.03 ± 0.05)×10–4 °C–1 for Hb and – (1.37 ± 0.07)×10–4 °C–1for HbA1c.The data obtained in the work can serve as a basis for further study of the optical properties of glycated hemoglobin and other glycated proteins.

Paper Details

Date Published: 17 May 2018
PDF: 8 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 1068540 (17 May 2018); doi: 10.1117/12.2307102
Show Author Affiliations
Ekaterina N. Lazareva, Immanuel Kant Baltic Federal Univ. (Russian Federation)
Saratov State Univ. (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Andrey Y. Zyubin, Immanuel Kant Baltic Federal Univ. (Russian Federation)
Ilya G. Samusev, Immanuel Kant Baltic Federal Univ. (Russian Federation)
Vasiliy A. Slezhkin, Immanuel Kant Baltic Federal Univ. (Russian Federation)
Kaliningrad State Technical Univ. (Russian Federation)
Vyacheslav I. Kochubey, Saratov State Univ. (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Valery V. Tuchin, Saratov State Univ. (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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