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Proceedings Paper

Optical monitoring of cell migration processes in a 3D scaffold
Author(s): Ines Delfino; Marianna Portaccio; Antonella D'Agostino; Marcella Cammarota; Ferdinando Campitiello; Chiara Schiraldi; Maria Lepore
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Paper Abstract

Three-dimensional (3D) polymeric scaffolds are utilized in tissue engineering to provide biomechanical support for the seeded cells until they are organized into a functional tissue. Studies of cell migration are important for understanding a variety of physiological and pathological processes in tissue regeneration. Optical techniques can be employed for monitoring cell migration processes in 3D scaffolds. In particular, two-photon microscopy (TPM) can be used for monitoring these processes for living cells also at considerable depths. In the present work, the cell migration process of alive chondrocytes labelled with vital PKH67 in 3D collagen-based scaffold has been monitored at different times from the seeding process. The results show that TPM can evidence the presence of living cells in different spatial regions whose localizations depend on the location and time elapsed from the seeding.

Paper Details

Date Published: 17 May 2018
PDF: 8 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853X (17 May 2018); doi: 10.1117/12.2306982
Show Author Affiliations
Ines Delfino, Univ. degli Studi della Tuscia (Italy)
Marianna Portaccio, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Antonella D'Agostino, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Marcella Cammarota, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Ferdinando Campitiello, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Chiara Schiraldi, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)
Maria Lepore, Univ. degli Studi della Campania Luigi Vanvitelli (Italy)


Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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