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Proceedings Paper

Optical UV-VIS-NIR spectroscopy of benign, dysplastic and malignant cutaneous lesions ex vivo
Author(s): E. Borisova; Ts. Genova-Hristova; P. Troyanova M.D.; I. Terziev M.D.; E. A. Genina; A. N. Bashkatov; O. Semyachkina-Glushkovskaya; V. Tuchin; L. Avramov
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Paper Abstract

Optical spectroscopic measurements with a few different modalities have been performed namely autofluorescence, transmission and diffuse reflectance spectroscopies in ultraviolet, visible and near-infrared spectral ranges.

The investigated samples were cutaneous tumours ex vivo, obtained after surgical removal and kept in a formalin solution and histological sections from biopsy tissue samples, which were routinely processed for histological analysis. Comparative spectral data for benign, dysplastic nevi and pigmented malignant melanoma lesions, as well as for nonmelanoma skin tumour – basal cell carcinoma, squamous cell carcinoma and benign non-melanin pigmented pathologies – heamangioma and seboreic veruca are presented in the current report.

Fluorescence spectra obtained reveal statistically significant differences between the different benign, dysplastic and malignant lesions by the level of emission intensity, as well by spectral shape, which are fingerprints applicable for differentiation algorithms. In reflectance and absorption modes the most significant differences are related to the influence of skin pigments – melanin and hemoglobin, less pronounced is the influence of structural proteins, such as collagen and keratin. Transmission spectroscopy mode gives complementary optical properties information about the tissue samples investigated to that one of reflectance and absorption spectroscopy.

Paper Details

Date Published: 17 May 2018
PDF: 6 pages
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853T (17 May 2018); doi: 10.1117/12.2306914
Show Author Affiliations
E. Borisova, Institute of Electronics (Bulgaria)
Saratov State Univ. (Russian Federation)
Ts. Genova-Hristova, Institute of Electronics (Bulgaria)
P. Troyanova M.D., Univ. Hospital “Tsaritsa Yoanna–ISUL” (Bulgaria)
I. Terziev M.D., Univ. Hospital "Tsaritsa Yoanna-ISUL" (Bulgaria)
E. A. Genina, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
A. N. Bashkatov, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
O. Semyachkina-Glushkovskaya, Saratov State Univ. (Russian Federation)
V. Tuchin, Saratov State Univ. (Russian Federation)
Tomsk State Univ. (Russian Federation)
L. Avramov, Institute of Electronics (Bulgaria)

Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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