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Proceedings Paper

Single-shot microsecond-resolved spectroscopy of the bacteriorhodopsin photocycle with quantum cascade laser frequency combs (Conference Presentation)
Author(s): Markus Geiser; Jessica L. Klocke; Markus Mangold; Pitt Allmendinger; Andreas Hugi; Pierre Jouy; Balint Horvath; Jérôme Faist; Tilman Kottke

Paper Abstract

Time-resolved vibrational spectroscopy is an important tool for understanding biological processes and chemical reaction pathways [1]. Today, all available methods to our knowledge require many repetitions of an experiment to acquire a microsecond time-res. mid-IR spectrum. We present the IRspectrometer, a quantum cascade laser dual frequency comb spectrometer [2-3]. It allows for parallel acquisition of hundreds of mid-infrared wavelengths with microsecond time resolution. The formation of the light-activated L, M and N-states in bacteriorhodopsin – which only have µs to ms lifetimes – has been recorded that show the infrared response of bacteriorhodopsin to 10 ns visible light pulses with microsecond time-resolution. The different wavelengths were all measured in parallel thanks to the dual-comb approach. The spectra as well as the kinetics show good agreement with those from step-scan FT-IR measurements. As a benchmark, the spectral signature of several intermediate states of the bacteriorhodopsin photocycle has been recorded in a single shot measurement. This approach greatly reduces the complexity of time-resolved bio-spectroscopy measurements in the mid-infrared which currently require many repetitions.

Paper Details

Date Published: 24 May 2018
Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106852G (24 May 2018); doi: 10.1117/12.2306831
Show Author Affiliations
Markus Geiser, IRsweep GmbH (Switzerland)
Jessica L. Klocke, Univ. Bielefeld (Germany)
Markus Mangold, IRsweep GmbH (Switzerland)
Pitt Allmendinger, IRsweep GmbH (Switzerland)
Andreas Hugi, IRsweep GmbH (Switzerland)
Pierre Jouy, IRsweep GmbH (Switzerland)
Balint Horvath, IRsweep GmbH (Switzerland)
Jérôme Faist, ETH Zurich (Switzerland)
Tilman Kottke, Univ. Bielefeld (Germany)

Published in SPIE Proceedings Vol. 10685:
Biophotonics: Photonic Solutions for Better Health Care VI
Jürgen Popp; Valery V. Tuchin; Francesco Saverio Pavone, Editor(s)

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